Direct identification of differentially expressed genes by cycle sequencing and cycle labelling using the differential display PCR primers
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چکیده
منابع مشابه
Direct identification of differentially expressed genes by cycle sequencing and cycle labelling using the differential display PCR primers.
Differential display PCR (DD-PCR) is an mRNA fingerprinting technique to identify differentially expressed genes by comparative display of arbitrarily amplified cDNA subsets. This attractively simple screening method was, however, followed by a labour intensive multistep identification procedure for DD-PCR products. In this report we demonstrate for the mouse mast cell protease 2 (MMCP-2) and t...
متن کاملRapid sequencing of unpurified PCR products by thermal asymmetric PCR cycle sequencing using unlabeled sequencing primers.
Numerous methods have been developed for direct sequencing of single or double-stranded DNA amplified by PCR, and most require template purification steps prior to sequencing to remove excess unincorporated primers and dNTPs. Purification methods can be cumbersome, laborious and time consuming, or yield low DNA recoveries. Strategies for direct sequencing of doublestranded DNA products without ...
متن کاملIdentification of differentially expressed genes in gauze-exposed omentum of dogs using differential display RT-PCR
Molecular mechanisms governing peritonitis caused by the presence of aseptic gauze have remained unclear. To identify the genes involved, sterile gauze-exposed omentum was collected at 0, 6, 12, 24, and 48 h intervals, and analyzed by differential display RT(reverse transcription)-PCR. Among over 1,200 bands, 230 bands were found differentially expressed. These bands represented the fragment si...
متن کاملDetection and differential display of expressed genes by DDRT-PCR.
~The first two authors contributed equally to the establishment of the method and to the results obtained. 4present address: Max-Planck-Gesellschaft, MaxDelbr~ick-Haus, 13122 Berlin-Buch, Germany. Analysis of gene expression is a central aim in most studies in molecular and cell biology. Interest in tissue-specific gene expression and, in particular, in changes in the expression patterns occurr...
متن کاملCycle Sequencing: Dideoxy-mediated Sequencing Reactions Using PCR and End-labeled Primers.
MATERIALS 5x Cycle-sequencing buffer AmpliTaq CS or other exonuclease-deficient versions of Taq DNA polymerase (5 units/μl) EDTA (0.05 M, pH 8.0) Formamide loading buffer Oligonucleotide Primers, radiolabeled at the 5' terminus with 33P or 32P To prepare end-labeled primers, please see Phosphorylating the 5' Termini of Oligonucleotides. Template DNAs Plasmids, cosmids, bacteriophage , and bacte...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1997
ISSN: 1362-4962
DOI: 10.1093/nar/25.11.2233